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Oxidative-Fermentative Glucose Medium, Semisolid, with NaCl (OF Glucose Medium, Semisolid with NaCl)

[所属分类:培养基配方] [发布时间:2021-12-8] [发布人:网站管理员2] [阅读次数:] [返回]

Oxidative-Fermentative

Glucose Medium, Semisolid, with NaCl

(OF Glucose Medium, Semisolid

with NaCl)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl..................................................................20.0g

Glucose .............................................................10.0g

Agar ....................................................................2.0g

Pancreatic digest of casein..................................2.0g

K2HPO4...............................................................0.3g

Bromthymol Blue dye.......................................0.08g

pH 6.8 ± 0.2 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 15 min

at 15 psi pressure–121°C. Pour into sterile Petri dish

es or leave in tubes.

Use: For differentiating halophilic Vibrio species

based upon determining the oxidative and fermenta

tive metabolism of glucose. Bacteria that ferment

glucose turn the medium yellow.