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Oxidation-Fermentation Medium, Hugh-Leifson’s (Hugh-Leifson’s Oxidation Fermentation Medium)

[所属分类:培养基配方] [发布时间:2021-12-8] [发布人:网站管理员2] [阅读次数:] [返回]

Oxidation-Fermentation

Medium, Hugh-Leifson’s

(Hugh-Leifson’s Oxidation

Fermentation Medium)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl....................................................................5.0g

Agar ....................................................................3.0g

Peptone ...............................................................2.0g

K2HPO4...............................................................0.3g

Carbohydrate solution................................. 100.0mL

Bromthymol Blue solution (0.2% ) .............. 15.0mL

pH 7.1 ± 0.2 at 25°C

Carbohydrate Solution:

Composition per 100.0mL:

Carbohydrate.....................................................10.0g

Preparation of Carbohydrate Solution: Add

carbohydrate to distilled/deionized water and bring

volume to 100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept carbohydrate solution, to distilled/deionized wa

ter and bring volume to 900.0mL. Mix thoroughly.

Gently heat and bring to boiling. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 45°–50°C.

Aseptically add 100.0mL of sterile carbohydrate so

lution. Mix thoroughly. Pour into sterile Petri dishes

or distribute into sterile tubes.

Use: For differentiating Gram-negative bacteria,

such as Vibrio species, based upon determining the

oxidative and fermentative metabolism of carbohy

drates. Bacteria that ferment the carbohydrate turn

the medium yellow.