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Nitrate Reduction Broth for Pseudomonas and Related Genera

[所属分类:培养基配方] [发布时间:2021-12-6] [发布人:网站管理员2] [阅读次数:] [返回]

Nitrate Reduction Broth for

Pseudomonas and Related Genera

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Peptone ...............................................................5.0g

NaCl....................................................................5.0g

Yeast extract........................................................2.0g

Beef extract.........................................................1.0g

NaNO3.................................................................0.1g

pH 7.4 ± 0.2 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Distribute into test tubes that con

tain an inverted Durham tube. Autoclave for 15 min

at 15 psi pressure–121°C.

Use: For the differentiation of members of the

Pseudomonadaceae based on their ability to reduce

nitrate to nitrite or form N2 gas. Test for nitrates with

sulfanilic acid and α-naphthylamine reagents. Bacte

ria that reduce nitrate to nitrite turn the reagents red

or pink.