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Nitrate Reduction Broth, Clark

[所属分类:培养基配方] [发布时间:2021-12-6] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Peptone .............................................................20.0g

KNO3 or NaNO3 .................................................2.0g

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Distribute into test tubes that con

tain an inverted Durham tube. Autoclave for 15 min

at 15 psi pressure–121°C.

Use: For the differentiation of a variety of Gram

negative bacteria based on their ability to reduce ni

trate to nitrite or form N2 gas. Test for nitrates with

sulfanilic acid and α-naphthylamine reagents. Bacte

ria that reduce nitrate to nitrite turn the reagents red

or pink.