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Nitrate Reduction Medium and Reagents (BAM M110)

[所属分类:培养基配方] [发布时间:2021-12-6] [发布人:网站管理员2] [阅读次数:] [返回]

Nitrate Reduction Medium

and Reagents

(BAM M110)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Peptone................................................................5.0g

Beef extract.........................................................3.0g

KNO3 ..................................................................1.0g

pH 7.0 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Distribute into tubes or flasks. Auto

clave for 15 min at 15 psi pressure–121°C.

Reagent A:

Composition per 100mL:

α-Naphthylamine................................................0.5g

Acetic acid, 5N............................................ 100.0mL

Caution: α-Naphthylamine is a carcinogen.

Preparation of Reagent A: Prepare 5N acetic

acid by adding distilled/deionized water to 28.7mL

glacial acetic acid to give a final volume of 100.0mL.

Add 0.5g α-naphthylamine to 100.0mL 5N acetic ac

id. Mix thoroughly.

Reagent B:

Composition per 100mL:

Sulfanilic acid .....................................................0.8g

Acetic acid, 5N............................................ 100.0mL

Preparation of Reagent B: Prepare 5N acetic

acid by adding distilled/deionized water to 28.7mL

glacial acetic acid to give a final volume of 100.0mL.

Add 0.8g sulfanilic acid to 100.0mL 5N acetic acid.

Mix thoroughly. Gently heat while mixing.

Reagent C:

Composition per 200mL:

α-Naphthol..........................................................1.0g

Acetic acid .................................................. 200.0mL

Preparation of Reagent C: Add 1.0g α-naphthol

to 200.0mL acetic acid. Mix thoroughly.

Zinc Powder:

Cadmium Reagent:

Preparation of Cadmium Reagent: Place zinc

rods in 20% solution of CdSO4. Allow to stand for

several hours. Draw off precipitated cadmium and

add to 1N HCl.

Use: For the differentiation of aerobic and facultative

Gram-negative microorganisms based on their ability to

reduce nitrate. Test for nitrates with sulfanilic acid and

α-naphthylamine reagents. Bacteria that reduce nitrate

to nitrite turn the reagents red or pink.

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Nitrate Reduction Medium and Reagents (BAM M110)