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Nitrate Reduction Broth

[所属分类:培养基配方] [发布时间:2021-12-6] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein................................13.0g

NaCl....................................................................5.0g

Yeast extract........................................................5.0g

Heart muscle, solids from infusion.....................2.0g

KNO3 or NaNO3 .................................................2.0g

pH 7.4 ± 0.2 at 25°C

Preparation of Medium: Dispense in 3.0mL

amounts into screw-capped tubes, add inverted vials,

and autoclave at 121°C for 15 min.

Use: For the differentiation of a variety of nonfer

menting Gram-negative bacteria based on their abili

ty to reduce nitrate to nitrite or form N2 gas. Test for

nitrates with sulfanilic acid and α-naphthylamine re

agents. Bacteria that reduce nitrate to nitrite turn the

reagents red or pink.