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Vitamin B6 Blood Agar (ATCC Medium 860)

[所属分类:培养基配方] [发布时间:2021-10-11] [发布人:网站管理员2] [阅读次数:] [返回]

Vitamin B6 Blood Agar

(ATCC Medium 860)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Pancreatic digest of casein.......................................................... 15.0g

Papaic digest of soybean meal...................................................... 5.0g

NaCl.............................................................................................. 5.0g

Sheep blood, defibrinated ........................................................50.0mL

pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components, except sheep blood, to

distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres

sure–121°C. Cool to 45°–50°C. Aseptically add sterile, defibrinated

sheep blood. Pour into sterile Petri dishes or distribute into sterile

tubes.

Use: For the cultivation and maintenance of fastidious microorganisms,

especially Streptococcus species.