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Viral Transport Medium (VTM)

[所属分类:培养基配方] [发布时间:2021-10-11] [发布人:网站管理员2] [阅读次数:] [返回]

Viral Transport Medium

(VTM)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 104.1mL:

Bovine serum albumin .................................................................. 0.5g

Veal infusion broth.................................................................100.0mL

Phenol Red.................................................................................0.4mL

Amphotericin B solution............................................................2.0mL

Gentamicin solution...................................................................1.0mL

Vancomycin solution..................................................................0.2mL

pH 7.4 ± 0.2 at 25°C

Veal Infusion Broth:

Composition per liter:

Veal, infusion from ................................................................... 500.0g

NaCl .............................................................................................. 5.0g

Pancreatic digest of casein............................................................ 5.0g

Peptic digest of animal tissue........................................................ 5.0g

Preparation of Veal Infusion Broth: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Dis

tribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–

121°C. Use freshly prepared solution.

Amphotericin B Solution:

Composition per 10.0mL:

Amphotericin B............................................................................. 2.5g

Preparation of Amphotericin B Solution: Add amphotericin B

to distilled/deionized water and bring volume to 10.0mL. Mix thor

oughly. Filter sterilize.

Gentamicin Solution:

Composition per 10.0mL:

Gentamicin.................................................................................... 0.5g

Preparation of Gentamicin Solution: Add gentamicin to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Filter sterilize.

Vancomycin Solution:

Composition per 10.0mL:

Vancomycin .................................................................................. 0.5g

Preparation of Vancomycin Solution: Add vancomycin to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Filter sterilize.

Preparation of Medium: To 100.0mL of sterile veal infusion

broth, aseptically add bovine serum albumin, Phenol Red, amphoteri

cin B solution, gentamicin solution, and vancomycin solution. Mix

thoroughly. Dispense 2.0mL of medium into serum vials. Store at 4°C

and use for up to 2 months.

Use: For the maintenance and transport of specimens suspected of

being virally infected.

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Viral Transport Medium (VTM)