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Vibrio vulnificus Agar (VVA) (BAM M190)

[所属分类:培养基配方] [发布时间:2021-10-11] [发布人:网站管理员2] [阅读次数:] [返回]

Vibrio vulnificus Agar

(VVA)

(BAM M190)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................ 30.0g

Agar ............................................................................................ 25.0g

Peptone ....................................................................................... 20.0g

Cellobiose solution ................................................................100.0mL

Dye solution.............................................................................10.0mL

pH 8.2 ± 0.2 at 25°C

Dye Solution:

Composition per 100.0mL:

Bromthymol Blue ......................................................................... 0.6g

Ethanol, 70%..........................................................................100.0mL

Preparation of Dye Solution: Add Bromthymol Blue to 100.0mL

of 70% ethanol. Mix thoroughly.

Cellobiose Solution:

Composition per 100.0mL:

Cellobiose ................................................................................... 10.0g

Preparation of Cellobiose Solution: Add cellobiose to distilled/

deionized water and bring volume to 100.0mL. Mix thoroughly. Gently

heat while mixing to dissolve the cellobiose. Cool. Filter sterilize.

Preparation of Medium: Add components, except cellobiose solu

tion, to distilled/deionized water and bring volume to 900.0mL. Mix

thoroughly. Gently heat until dissolved. Adjust pH to 8.2. Autoclave

for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add

100.0mL sterile cellobiose solution. Mix thoroughly and pour into ster

ile Petri dishes. Final color of medium should be light blue.

Use: For the detection of Vibrio vulnificus from seafoods.