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Vibrio vallismortis Medium

[所属分类:培养基配方] [发布时间:2021-10-11] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................ 25.0g

MgSO4·7H2O................................................................................ 9.6g

MgCl2·6H2O ................................................................................. 7.0g

Glucose ......................................................................................... 5.0g

KCl................................................................................................ 3.8g

Yeast extract.................................................................................. 1.0g

CaCl2·2H2O .................................................................................. 0.5g

K2HPO4·3H2O .............................................................................. 0.4g

NaHCO3 solution.....................................................................20.0mL

pH 7.0 ± 0.2 at 25°C

NaHCO3 Solution:

Composition per 20.0mL:

NaHCO3........................................................................................ 3.0g

Preparation of NaHCO3 Solution: Add NaHCO3 to distilled/de

ionized water and bring volume to 20.0mL. Mix thoroughly. Filter ster

ilize.

Preparation of Medium: Add components, except NaHCO3 solu

tion, to distilled/deionized water and bring volume to 980.0mL. Mix

thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to

room temperature. Aseptically add 20.0mL of sterile NaHCO3 solu

tion. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation of Vibrio vallismortis.