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Vibrio parahaemolyticus Sucrose Agar (VPSA)

[所属分类:培养基配方] [发布时间:2021-10-11] [发布人:网站管理员2] [阅读次数:] [返回]

Vibrio parahaemolyticus Sucrose Agar

(VPSA)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl ............................................................................................ 30.0g

Agar ............................................................................................ 15.0g

Sucrose........................................................................................ 10.0g

Yeast extract.................................................................................. 7.0g

Tryptose ........................................................................................ 5.0g

Pancreatic digest of casein............................................................ 5.0g

Bile salts No. 3.............................................................................. 1.5g

Bromthymol Blue ..................................................................... 0.025g

pH 8.6 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Do not autoclave. Cool to 50°C. Pour into sterile Petri dishes

in 20.0mL volumes. Allow plates to dry before using.

Use: For the isolation, cultivation, and differentiation of Vibrio para

haemolyticus from seafood. Vibrio parahaemolyticus and Vibrio vul

nificus appear as blue to green colonies. Other Vibrio species appear as

yellow colonies.