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Vibrio parahaemolyticus Agar (VP Agar)

[所属分类:培养基配方] [发布时间:2021-10-9] [发布人:网站管理员2] [阅读次数:] [返回]

Vibrio parahaemolyticus Agar

(VP Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 20.0g

NaCl ............................................................................................ 20.0g

Sucrose........................................................................................ 20.0g

Sodium citrate ............................................................................. 10.0g

Na2S2O3·5H2O ............................................................................ 10.0g

Peptone........................................................................................ 10.0g

Sodium taurocholate ..................................................................... 5.0g

Yeast extract.................................................................................. 5.0g

Sodium lauryl sulfate .................................................................... 0.2g

Bromthymol Blue ....................................................................... 0.04g

Thymol Blue ............................................................................... 0.04g

pH 8.6 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Do not autoclave. Pour into sterile Petri dishes.

Use: For the isolation, cultivation, enumeration, and presumptive

identification of coliforms in milk, food, and other specimens of san

itary significance. For the enumeration of bacteria in cheese, espe

cially Pseudomonas fragi, Pseudomonas viscosa, and Alcaligenes

metalcaligenes. Sucrose-fermenting bacteria appear as yellow colo

nies with pale yellow peripheries. Sucrose-nonfermenting bacteria

appear as mucoid, green colonies with a dark green center.