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Veillonella Selective Medium

[所属分类:培养基配方] [发布时间:2021-10-9] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein............................................................ 5.0g

Yeast extract.................................................................................. 3.0g

Tween™ 80................................................................................... 1.0g

Sodium thioglycolate.................................................................. 0.75g

Sodium lactate (50% solution).................................................25.0mL

Streptomycin solution ..............................................................10.0mL

pH 6.6 ± 0.2 at 25°C

Streptomycin Solution:

Composition per 10.0mL:

Streptomycin..............................................................................5.0mg

Preparation of Streptomycin Solution: Add streptomycin to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Filter sterilize.

Preparation of Medium: Add components, except streptomycin

solution, to distilled/deionized water and bring volume to 990.0mL.

Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 6.6 with

K2CO3. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–

50°C. Aseptically add sterile streptomycin solution. Mix thoroughly.

Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation of Veillonella species.