培养基配方

您现在的位置：网站首页 >> 培养基配方

V-8™ Rye Agar

[所属分类:培养基配方] [发布时间:2021-10-8] [发布人:网站管理员2] [阅读次数:] [返回]

V-8™ Rye Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1050.0mL:

Agar ............................................................................................ 20.0g

CaCO3 .......................................................................................... 0.2g

Rye broth.......................................................................................1.0L

V-8™ canned vegetable juice ..................................................50.0mL

Rye Broth:

Composition per liter:

Whole rye grains......................................................................... 50.0g

Preparation of Rye Broth: Soak the rye grains in 1.1L of distilled/

deionized water for 24–36 hr at 24°C. Autoclave at 15 psi pressure–

121°C for 30 min. Filter through four layers of cheesecloth. Bring the

final filtrate volume to 1.0L with distilled/deionized water.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15

psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Phytophthora infestans.