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V-8™ Juice Seawater Agar

[所属分类:培养基配方] [发布时间:2021-10-8] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

CaCO3 ........................................................................................... 3.0g

Artificial seawater..................................................................800.0mL

V-8™ canned vegetable juice, unsalted................................ 200.0mL

pH 7.0 ± 0.2 at 25°C

Artificial Seawater:

Composition per liter:

NaCl ............................................................................................ 20.0g

MgCl2·6H2O................................................................................ 5.38g

MgSO4·7H2O .............................................................................. 6.78g

KCl.............................................................................................. 0.72g

NaHCO3 ....................................................................................... 0.2g

CaCl2·2H2O................................................................................... 1.4g

Preparation of Artificial Seawater: Add components to distilled/

deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Combine components. Mix thoroughly.

Adjust pH to 7.0. Gently heat and bring to boiling. Distribute into tubes

or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into

sterile Petri dishes or leave in tubes.

Use: For the cultivation of Halophytophthora masteri and Halophy

tophthora tartarea.