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Trebouxia Agar

[所属分类:培养基配方] [发布时间:2021-9-18] [发布人:网站管理员2] [阅读次数:] [返回]

Trebouxia Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Bristol's solution ....................................................................850.0mL

Soil extract.............................................................................140.0mL

Glucose ....................................................................................... 20.0g

Agar ............................................................................................ 15.0g

Proteose peptone......................................................................... 10.0g

Bristol's Solution:

Composition per 1000.1mL:

NaNO3 solution........................................................................... 10.0g

KH2PO4 solution........................................................................... 7.0g

K2HPO4 solution........................................................................... 3.0g

MgSO4·7H2O solution .................................................................. 3.0g

CaCl2 solution ............................................................................... 1.0g

NaCl solution ................................................................................ 1.0g

FeCl3 solution ............................................................................0.1mL

NaNO3 Solution:

Composition per 400.0mL:

NaNO3......................................................................................... 10.0g

Preparation of NaNO3 Solution: Add NaNO3 to distilled/deion

ized water and bring volume to 400.0mL. Mix thoroughly.

CaCl2 Solution:

Composition per 400.0mL:

CaCl2............................................................................................. 1.0g

Preparation of CaCl2 Solution: Add CaCl2 to distilled/deionized

water and bring volume to 400.0mL. Mix thoroughly.

MgSO4·7H2O Solution:

Composition per 400.0mL:

MgSO4·7H2O ................................................................................ 3.0g

Preparation of MgSO4·7H2O Solution: Add MgSO4·7H2O to

distilled/deionized water and bring volume to 400.0mL. Mix thorough

ly.

K2HPO4 Solution:

Composition per 400.0mL:

K2HPO4......................................................................................... 3.0g

Preparation of K2HPO4 Solution: Add K2HPO4 to distilled/de

ionized water and bring volume to 400.0mL. Mix thoroughly.

KH2PO4 Solution:

Composition per 400.0mL:

KH2PO4......................................................................................... 7.0g

Preparation of KH2PO4 Solution: Add KH2PO4 to distilled/de

ionized water and bring volume to 400.0mL. Mix thoroughly.

NaCl Solution:

Composition per 400.0mL:

NaCl .............................................................................................. 1.0g

Preparation of NaCl Solution: Add NaCl to distilled/deionized

water and bring volume to 400.0mL. Mix thoroughly.

FeCl3 Solution:

Composition per 100.0mL:

FeCl3 ............................................................................................. 1.0g

Preparation of FeCl3 Solution: Add FeCl3 to distilled/deionized

water and bring volume to 100.0mL. Mix thoroughly.

Preparation of Bristol's Solution: Add 10.0mL of NaNO3 solu

tion, 10.0mL of CaCl2 solution, 10.0mL of MgSO4·7H2O solution,

10.0mL of NaNO3 solution, 10.0mL of K2HPO4 solution, 10.0mL of

KH2PO4 solution, and 10.0mL of NaCl solution to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Add 0.1mL of FeCl3

solution. Mix thoroughly.

Soil Extract:

Composition per 200.0mL:

African Violet soil....................................................................... 77.0g

Na2CO3 ......................................................................................... 0.2g

Preparation of Soil Extract: Add components to distilled/deion

ized water and bring volume to 200.0mL. Mix thoroughly. Autoclave

for 60 min at 15 psi pressure–121°C. Filter through Whatman #1 filter

paper.

Preparation of Medium: Combine components. Mix thoroughly.

Gently heat and bring to boiling. Distribute into tubes or flasks. Auto

clave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes

or leave in tubes.

Use: For the cultivation of Brachiomonas submarina and Trebouxia

magna.

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Trebouxia Agar