培养基配方

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Transport Medium Stuart

[所属分类:培养基配方] [发布时间:2021-9-18] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Sodium glycerophosphate........................................................... 10.0g

Agar .............................................................................................. 3.0g

Sodium thioglycolate.................................................................... 0.9g

CaCl2·2H2O .................................................................................. 0.1g

Methylene Blue.......................................................................... 2.0mg

pH 7.3 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di

agnostic Systems.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat while

stirring and bring to boiling. Distribute into screw-capped tubes or vi

als. Fill tubes nearly to capacity. Leave only enough space so that when

a small swab is introduced the tube does not overflow. Autoclave for

10 min at 15 psi pressure–121°C. Tighten caps on tubes.

Use: For the transportation of swab specimens for the recovery of a

wide variety of microorganisms, including Neisseria gonorrhoeae.