Pelobacter acidigallici Medium

[所属分类:培养基配方] [发布时间:2021-7-1] [发布人:网站管理员2] [阅读次数:] [返回]

Pelobacter acidigallici Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1001.0mL:

NaCl............................................................................................20.0g
MgCl 2 ·6H 2 O..................................................................................3.0g
KCl................................................................................................0.5g
NH 4 Cl .........................................................................................0.25g
KH 2 PO 4 .........................................................................................0.2g
CaCl 2 ·2H 2 O.................................................................................0.15g
Resazurin ...................................................................................1.0mg
NaHCO 3  solution .....................................................................10.0mL
Na 2 S·9H 2 O solution.................................................................10.0mL
Gallic acid solution..................................................................10.0mL
Trace elements solution SL-10 ..................................................1.0mL
pH 7.2 ± 0.2 at 25°C
NaHCO 3 Solution:
Composition per 10.0mL:
NaHCO 3 ........................................................................................2.5g
Preparation of NaHCO 3 Solution:  Add NaHCO 3 to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster-
ilize. Sparge with 80% N 2 + 20% CO 2 .
Na 2 S·9H 2 O Solution:
Composition per 10.0mL:
Na 2 S·9H 2 O..................................................................................0.36g
Preparation of Na 2 S·9H 2 O Solution:  Add Na 2 S·9H 2 O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Sparge with 100% N 2 . Autoclave for 15 min at 15 psi pressure–121°C.
Gallic Acid Solution:
Composition per 10.0mL:
Gallic acid.....................................................................................1.0g
Preparation of Gallic Acid Solution:  Add gallic acid to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize. Sparge with 80% N 2 + 20% CO 2 .
Trace Elements Solution SL-10:
Composition per liter:
FeCl 2 ·4H 2 O...................................................................................1.5g
CoCl 2 ·6H 2 O................................................................................0.19g
MnCl 2 ·4H 2 O ..........................................................................100.0mg
ZnCl 2 ........................................................................................70.0mg
Na 2 MoO 4 ·2H 2 O.......................................................................36.0mg
NiCl 2 ·6H 2 O..............................................................................24.0mg
H 3 BO 3 ........................................................................................6.0mg
CuCl 2 ·2H 2 O...............................................................................2.0mg
HCl (25% solution)..................................................................10.0mL
Preparation of Trace Elements Solution SL-10:  Add FeCl 2 ·4H 2 O
to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized
water and bring volume to 1.0L. Add remaining components. Mix thor-
oughly.
Preparation of Medium: Prepare and dispense medium under 80%
H 2 + 20% CO 2 . Add components, except NaHCO 3  solution,
Na 2 S·9H 2 O solution, gallic acid solution, and trace elements solution
SL-10, to distilled/deionized water and bring volume to 970.0mL. Mix
thoroughly. Sparge with 80% N 2 + 20% CO 2 . Autoclave for 15 min at
15 psi pressure–121°C. Aseptically and anaerobically add 10.0mL of
sterile NaHCO 3  solution, 10.0mL of sterile Na 2 S·9H 2 O solution,
10.0mL of sterile gallic acid solution, and 1.0mL of sterile trace ele-
ments solution SL-10 or, using a syringe, inject the appropriate amount
of sterile NaHCO 3 solution, sterile Na 2 S·9H 2 O solution, sterile gallic
acid solution, and sterile trace elements solution SL-10 into individual
tubes containing medium.
Use: For the cultivation and maintenance of Pelobacter acidigallici.