Payne, Seghal, and Gibbons Medium (DSMZ Medium 1160)

[所属分类:培养基配方] [发布时间:2021-6-30] [发布人:网站管理员2] [阅读次数:] [返回]

Payne, Seghal, and Gibbons Medium
(DSMZ Medium 1160)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl..........................................................................................250.0g
MgSO 4 ·7H 2 O..............................................................................20.0g
Yeast extract................................................................................10.0g
Casamino acids.............................................................................7.5g
Trisodium citrate...........................................................................3.0g
KCl................................................................................................2.0g
FeCl 2 ·4H 2 O..............................................................................36.0mg
MnCl 2 ·4H 2 O ............................................................................0.36mg
pH 7.4 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.4.
Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C.
Use: For the cultivation of Haloarcula californiae, Haloarcula hispan-
ica, Haloarcula japonica, Haloarcula marismortui, Haloarcula sinai-
iensis, Haloarcula vallismortis, Halobacterium cutirubrum, Halobacte-
rium distributum, Halobacterium lacusprofundi, Halobacterium
saccharovorum, Haloferax gibbonsii, Haloferax mediterranei,Halobac-
terium salinarium, Halobacterium simoncinii, Halobacterium species,
Halobacterium trapanicum, Halobacterium volcanii, Halococcus mor-
rhuae, Halococcus saccharolyticus, Halococcus species, Halococcus
turkmenicus, Haloferax denitrificans, Halomonas elongata, and Salinic-
occus roseus. For the cultivation of Natrinema pallidum.