Pasteurella multocida Selective Medium

[所属分类:培养基配方] [发布时间:2021-6-30] [发布人:网站管理员2] [阅读次数:] [返回]

Pasteurella multocida Selective Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1020.0mL:

Tryptose agar with peptic digest of blood.....................................1.0L
Antibiotic solution ...................................................................10.0mL
K 2 TeO 3 solution.......................................................................10.0mL
pH 7.2 ± 0.2 at 25°C
Tryptose Agar with Peptic Digest of Blood:
Composition per liter:
Agar ............................................................................................15.0g
Pancreatic digest of casein..........................................................10.0g
Peptic digest of animal tissue......................................................10.0g
NaCl..............................................................................................5.0g
Glucose.........................................................................................1.0g
Peptic digest of blood ..............................................................50.0mL
Preparation of Tryptose Agar with Peptic Digest of Blood:
Add components to distilled/deionized water and bring volume to
950.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically
add peptic digest of blood. Mix thoroughly.
Antibiotic Solution:
Composition per 10.0mL:
Actidione (cycloheximide) ...........................................................0.1g
Novobiocin .................................................................................0.01g
Erythrocin..................................................................................5.0mg
Preparation of Antibiotic Solution: Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
K 2 TeO 3  Solution:
Composition per 10.0mL:
K 2 TeO 3 .......................................................................................5.0mg
Preparation of K 2 TeO 3 Solution: Add K 2 TeO 3 to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly. Filter steril-
ize.
Caution: Potassium tellurite is toxic.
Preparation of Medium: To 1.0L of cooled, sterile tryptose agar with
peptic digest of blood, aseptically add 10.0mL of sterile antibiotic solution
and 10.0mL of sterile K 2 TeO 3 solution. Mix thoroughly. Pour into sterile
Petri dishes or distribute into sterile tubes.
Use: For the selective cultivation of Pasteurella multocida.