PA HiVeg Broth

[所属分类:培养基配方] [发布时间:2021-6-29] [发布人:网站管理员2] [阅读次数:] [返回]

PA HiVeg Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Plant hydrolysate No. 1...............................................................9.83g
Lactose........................................................................................7.46g
Plant peptone.................................................................................5.0g
Plant extract ..................................................................................3.0g
NaCl............................................................................................2.46g
KH 2 PO 4 .......................................................................................1.35g
K 2 HPO 4 .......................................................................................1.35g
Sodium lauryl sulfate..................................................................0.05g
Bromcresol Purple ..................................................................0.8.5mg
pH 6.8 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.5. Au-
toclave for 15 min at 15 psi pressure–121°C. Cool to 55°–60°C. Read-
just pH to 7.1. Mix thoroughly. Pour into 50mm × 12mm Petri dishes
in 3.0mL volumes.
Use:  For the cultivation and estimation of numbers of Pseudomonas
aeruginosa in water by the membrane filter method. For the detection
of presence and absence of coliform bacteria in water from treatment
plants or distribution systems.