P-2 Medium

[所属分类:培养基配方] [发布时间:2021-6-29] [发布人:网站管理员2] [阅读次数:] [返回]

P-2 Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Yeast extract..................................................................................5.0g
K 2 HPO 4 .........................................................................................3.0g
KH 2 PO 4 .........................................................................................2.0g
NH 4 Cl ...........................................................................................2.0g
L -Cysteine·HCl..............................................................................0.5g
Na 2 S·9H 2 O....................................................................................0.5g
MgCl 2 ·6H 2 O..................................................................................0.2g
CaCl 2 ·2H 2 O.................................................................................0.05g
Resazurin ...................................................................................0.5mg
Glucose solution ...........................................................................5.0g
pH 7.0–7.2 at 25°C
Glucose Solution:
Composition per 100.0mL:
D -Glucose......................................................................................5.0g
Preparation of Glucose Solution:  Add glucose to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Sparge with
100% N 2 . Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Prepare and dispense medium under
100% N 2 . Add components, except glucose solution, to distilled/deion-
ized water and bring volume to 900.0mL. Mix thoroughly. Adjust pH
to 7.0–7.2. Sparge with 100% N 2 . Autoclave for 15 min at 15 psi pres-
sure–121°C. Aseptically and anaerobically add 100.0mL of sterile glu-
cose solution. Mix thoroughly. Aseptically and anaerobically distribute
into sterile tubes or bottles.
Use: For the cultivation of Clostridium uzonii, Thermoanaerobium
crenophilum, and Thermoanaerobium olidum.