Oxytetra Glucose Yeast Agar Base (OGYE Agar Base)

[所属分类:培养基配方] [发布时间:2021-6-29] [发布人:网站管理员2] [阅读次数:] [返回]

Oxytetra Glucose Yeast Agar Base
(OGYE Agar Base)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose.......................................................................................20.0g
Agar............................................................................................12.0g
Yeast extract..................................................................................5.0g
Oxytetracycline solution..........................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Source: This medium, without oxytetracycline solution, is available
as a premixed powder from HiMedia.
Oxytetracycline Solution:
Composition per 10.0mL:
Oxytetracycline.............................................................................0.1g
Tris(hydroxymethyl) aminomethane buffer (0.1M, pH 7.0)....10.0mL
Preparation of Oxytetracycline Solution: Add oxytetracycline
to 10.0mL of Tris buffer. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except oxytetracycline
solution, to distilled/deionized water and bring volume to 990.0mL.
Mix thoroughly. Gently heat and bring to boiling. Autoclave for 10 min
at 10 psi pressure–115°C. Cool to 45°–50°C. Aseptically add sterile
oxytetracycline solution. Mix thoroughly. Pour into sterile Petri dishes
or distribute into sterile tubes.
Use: For the isolation, enumeration, and cultivation of yeasts and other
fungi from foods.