Oxidative-Fermentative Test Medium (OF Test Medium)

[所属分类:培养基配方] [发布时间:2021-6-29] [发布人:网站管理员2] [阅读次数:] [返回]

Oxidative-Fermentative Test Medium
(OF Test Medium)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl..............................................................................................5.0g
Agar..............................................................................................3.0g
Peptone .........................................................................................2.0g
K 2 HPO 4 .........................................................................................0.3g
Bromthymol Blue.......................................................................0.03g
Carbohydrate solution............................................................100.0mL
Carbohydrate Solution:
Composition per 100.0mL:
Carbohydrate...............................................................................10.0g
Preparation of Carbohydrate Solution: Add carbohydrate to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components, except carbohydrate
solution, to distilled/deionized water and bring volume to 1.0L. Mix
thoroughly. Gently heat and bring to boiling. Distribute into tubes in
3.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C. Aseptically add 0.3mL of sterile carbohydrate solution to
each tube. Mix thoroughly.
Use: For the cultivation and differentiation of a variety of microorgan-
isms based on their ability to ferment a specific carbohydrate. Bacteria
that ferment the specific carbohydrate turn the medium yellow.