Oxalobacter Medium

[所属分类:培养基配方] [发布时间:2021-6-29] [发布人:网站管理员2] [阅读次数:] [返回]

Oxalobacter Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1001.0mL:

Sodium oxalate ...........................................................................10.0g
Na 2 CO 3 ........................................................................................4.0g
Yeast extract..................................................................................1.0g
Sodium acetate............................................................................0.82g
L -Cysteine·HCl..............................................................................0.5g
(NH 4 ) 2 SO 4 .....................................................................................0.5g
K 2 HPO 4 .......................................................................................0.25g
KH 2 PO 4 .......................................................................................0.25g
MgSO 4 ·7H 2 O............................................................................0.025g
Resazurin ...................................................................................1.0mg
Trace elements solution SL-10 ..................................................1.0mL
pH 6.8 ± 0.2 at 25°C
Trace Elements Solution SL-10:
Composition per liter:
FeCl 2 ·4H 2 O...................................................................................1.5g
CoCl 2 ·6H 2 O ...........................................................................190.0mg
MnCl 2 ·4H 2 O...........................................................................100.0mg
ZnCl 2 ........................................................................................70.0mg
Na 2 MoO 4 ·2H 2 O .......................................................................36.0mg
NiCl 2 ·6H 2 O..............................................................................24.0mg
H 3 BO 3 ........................................................................................6.0mg
CuCl 2 ·2H 2 O ...............................................................................2.0mg
HCl (25% solution)..................................................................10.0mL
Preparation of Trace Elements Solution SL-10: Add FeCl 2 ·4H 2 O
to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized
water and bring volume to 1.0L. Add remaining components. Mix thor-
oughly. Sparge with 100% N 2 . Autoclave for 15 min at 15 psi pressure–
121°C.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Sparge with 100%
CO 2 . Anaerobically distribute into tubes or flasks. Autoclave for 15
min at 15 psi pressure–121°C.
Use: For the cultivation of Aureobacterium testaceum and Oxalo-
bacter formigenes.