Oxalate Utilization Medium

[所属分类:培养基配方] [发布时间:2021-6-29] [发布人:网站管理员2] [阅读次数:] [返回]

Oxalate Utilization Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................12.0g
Potassium oxalate .........................................................................1.0g
NaCl..............................................................................................1.0g
(NH 4 ) 2 HPO 4 ..................................................................................1.0g
KH 2 PO 4 .........................................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.2g
CaCl 2 solution..........................................................................80.0mL
pH 7.0 ± 0.2 at 25°C
CaCl 2 Solution:
Composition per 100.0mL:
CaCl 2 ...........................................................................................1.47g
Preparation of CaCl 2 Solution: Add CaCl 2 to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Gently heat until
dissolved. Filter sterilize.
Preparation of Medium: Add components, except CaCl 2 solution,
to distilled/deionized water and bring volume to 920.0mL. Mix thor-
oughly. Gently heat and bring to boiling. Distribute into flasks in
92.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C. Aseptically add 8.0mL of sterile CaCl 2 solution to each
flask. A fine precipitate of calcium oxalate will form. Mix thoroughly.
Pour into sterile Petri dishes. Swirl flask while dispensing agar to dis-
perse precipitate evenly.
Use: For the cultivation and differentiation of streptomycetes based on
oxalate utilization. Bacteria that utilize oxalate turn the medium dark
blue.