HiCrome Nickels and Leesment Medium

[所属分类:培养基配方] [发布时间:2021-4-8] [发布人: ] [阅读次数:] [返回]

HiCrome Nickels and Leesment Medium

培养基配方

Composition per liter:
Casein enzymatic hydrolysate ....................................................18.0g
Agar............................................................................................15.0g
Calcium lactate pentahydrate........................................................8.0g
Tricalcium dicitrate tetrahydrate.................................................6.65g
Yeast extract..................................................................................4.5g
Glucose.........................................................................................4.5g
Lactose..........................................................................................4.5g
NaCl..............................................................................................3.6g
Gelatin.........................................................................................2.25g
Trisodium citrate dihydrate...........................................................1.8g
Carboxymethyl cellulose..............................................................0.4g
Chromogenic substrate (X-gal).....................................................0.2g
Selective supplement solution .................................................10.0mL
pH 6.65 ± 0.05 at 25°C
Source: This medium is available from HiMedia.
Selective Supplement Solution:
Composition per 10.0mL:
Vancomycin..................................................................................0.2g
Preparation of Selective Supplement Solution:  Add vancomycin to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except selective supplement solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add selective supplement solution. Mix thoroughly. Pour into Petri dishes or aseptically distribute into sterile tubes.
Use: For the enumeration of citrate-fermenting lactic acid bacteria from milk.