HiCrome™ M-TEC Agar

[所属分类:培养基配方] [发布时间:2021-4-8] [发布人: ] [阅读次数:] [返回]

HiCrome™ M-TEC Agar

培养基配方

Composition per liter:
Agar ............................................................................................15.0g
Lactose........................................................................................10.0g
NaCl..............................................................................................7.5g
Proteose peptone...........................................................................5.0g
K 2 HPO 4 .........................................................................................3.3g
Yeast extract..................................................................................3.0g
KH 2 PO 4 .........................................................................................1.0g
Chromogen....................................................................................0.5g
Sodium lauryl sulfate....................................................................0.2g
Sodium deoxycholate....................................................................0.1g
pH 7.3 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium: Add components to distilled/deionizedwater and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes.
Use:  For the differentiation and enumeration of thermotolerant E. coli from water by the membrane filtration method.