Erwinia amylovora Selective Medium

[所属分类:培养基配方] [发布时间:2020-7-8] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Erwinia amylovora Selective Medium

培养基配方
Composition per liter:
Agar........................................................................20.0g
Mannitol....................................................................10.0g
L- Asparagine................................................................3.0g
Sodium taurocholate..........................................................2.5g
K2HPO4 ......................................................................2.0g
Nicotinic acid...............................................................0.5g
MgSO4 ·7H2O.................................................................0.2g
Nitrilotriacetic acid......................................................10.0mL
Actidione (cycloheximide) solution.........................................10.0mL
Bromthymol Blue ............................................................9.0mL
Neutral Red.................................................................2.5mL
TlNO3 solution.............................................................1.75mL
Tergitol™ 7.................................................................0.1mL
pH 7.2–7.3 at 25°C
Cycloheximide Solution:
Composition per 10.0mL:
Cycloheximide...............................................................0.05g
Preparation of Cycloheximide Solution: Add cycloheximide to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
TlNO3 Solution:
Composition per 10.0mL:
TlNO3 .......................................................................0.1g
Preparation of TlNO3 Solution: Add TlNO3 to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly. Filter steril-
ize.
Preparation of Medium: Add components, except TlNO3 solution
and cycloheximide solution, to distilled/deionized water and bring vol-
ume to 988.25mL. Mix thoroughly. Adjust pH to 7.2–7.3. Gently heat
and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C.
Cool to 45°–50°C. Aseptically add 1.75mL of sterile TlNO 3 solution
and 10.0mL of sterile cycloheximide solution. Mix thoroughly. Pour
into sterile Petri dishes or distribute into sterile tubes.
Use: For the isolation and cultivation of Erwinia amylovora.