​Dulcitol Selenite Broth (Selenite-F Broth with Dulcitol)

[所属分类:培养基配方] [发布时间:2020-6-29] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Dulcitol Selenite Broth
(Selenite-F Broth with Dulcitol)

培养基配方
Composition per liter:
NaH2PO4 ......................................................................10.0g
Peptic digest of animal tissue.................................................5.0g
Dulcitol ......................................................................4.0g
HNaO3Se........................................................................4.0g
pH 7.0 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Caution: Sodium hydrogen selenite is a very toxic, corrosive agent
and causes teratogenicity. Upon contact with skin, wash immediately
with a lot of water.
Preparation of Medium: Add sodium hydrogen selenite to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Add
remaining components. Mix thoroughly. Gently heat if needed to get
all compoents to dissolve. Distribute into tubes or flasks. Sterilize in a
boiling water bath or free flowing steam for 10 min. Excessive heating
is detrimental. Do not autoclave.
Use: For the selective enrichment of Salmonella species.