Dulaney Slants

[所属分类:培养基配方] [发布时间:2020-6-29] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Dulaney Slants

培养基配方
Composition per liter:
Egg yolks.................................................................50.0mL
Locke solution ...........................................................50.0mL
Locke Solution:
Composition per 100.0mL:
NaCl.......................................................................0.9g
Glucose...................................................................0.25g
KCl......................................................................0.042g
CaCl2 ·2H2O.............................................................0.024g
Na2CO3 ...................................................................0.02g
Preparation of Locke Solution:  Add components to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium:  Aseptically remove the yolks from 5–8
day old hen egg embryos. Add an equal volume of sterile Locke solution
containing sterile glass beads. Mix thoroughly to homogenize. Asepti-
cally distribute into sterile tubes. Inspissate tubes in a slanted position at
80°C (moist heat) for 15 min.
Use: For the cultivation of Calymmatobacter granulomatis from clin-
ical specimens.