Dubos Salts Agar with Yeast Extract

[所属分类:培养基配方] [发布时间:2020-6-29] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Dubos Salts Agar with Yeast Extract

培养基配方
Composition per liter:
Agar ......................................................................15.0g
K2HPO4 .....................................................................1.0g
Yeast extract...............................................................0.5g
KCl.........................................................................0.5g
MgSO4 ·7H2O................................................................0.5g
NaNO3 ......................................................................0.5g
FeSO4 ·7H2O ..............................................................0.01g
Filter paper strips, sterile ...........................................variable
pH 7.2 ± 0.2 at 25°C
Preparation of Medium: Add components, except filter paper
strips, to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Pour into sterile Petri dishes. Aseptically place sterile
filter paper strips onto the surface of the solidified medium.
Use:  For the cultivation and maintenance of Cellulomonas species and
Cellvibrio species.