Dubos Salts Agar with 1% Sodium Chloride

[所属分类:培养基配方] [发布时间:2020-6-29] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Dubos Salts Agar with 1% Sodium Chloride

培养基配方
Composition per liter:
Agar.........................................................................15.0g
NaCl.........................................................................10.0g
K2HPO4 .......................................................................1.0g
KCl...........................................................................0.5g
MgSO4 ·7H2O..................................................................0.5g
NaNO3 ........................................................................0.5g
FeSO4 ·7H2O................................................................10.0mg
Filter paper strips..............................................1 strip per tube
pH 7.2 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes. Autoclave for 15 min at 15 psi pres-
sure–121°C. Allow tubes to cool in a slanted position. Aseptically add
a strip of sterile filter paper to the surface of each slant.
Use: For the cultivation of Cytophaga species.