​Desulfovibrio Medium

[所属分类:培养基配方] [发布时间:2020-6-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Desulfovibrio Medium

培养基配方
Composition per liter:
Solution A...................................................................980.0mL
Solution B....................................................................10.0mL
Solution C....................................................................10.0mL
pH 7.8 ± 0.2 at 25°C
Solution A:
Composition per 980.0mL:
Choline·HCl....................................................................5.0g
K2HPO4 .........................................................................4.0g
MgSO4 ·7H2O....................................................................2.0g
Na2SO4 .........................................................................1.0g
NH4Cl ..........................................................................1.0g
Yeast extract...................................................................1.0g
CaCl2 ·2H2O ...................................................................0.1g
Resazurin .....................................................................1.0mg
Preparation of Solution A:  Add components to distilled/deionized
water and bring volume to 980.0mL. Mix thoroughly. Gently heat and
bring to boiling. Continue boiling for 3–4 min. Allow to cool to room
temperature while gassing under 100% N2 .
Solution B:
Composition per 10.0mL:
FeSO4 ·7H2O....................................................................0.5g
Preparation of Solution B:  Add components to distilled/deionized
water and bring volume to 10.0mL. Mix thoroughly. Gas under 100%
N2 . Autoclave for 15 min at 15 psi pressure–121°C.
Solution C:
Composition per 10.0mL:
Ascorbic acid...................................................................0.1g
Sodium thioglycolate............................................................0.1g
Preparation of Solution C:  Add components to distilled/deionized
water and bring volume to 10.0mL. Mix thoroughly. Gas under 100%
N2 . Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Dissolve the ingredients of each solution
in the appropriate quantities of water. Bring solution A to a boil for a
few minutes, then cool to room temperature while gassing with oxy-
gen-free N2 gas. Add solutions B and C, adjust pH to 7.8 with NaOH,
and distribute under N2 in anaerobic tubes. During distribution, contin-
uously swirl the medium to keep the grey precipitate suspended. Auto-
clave for 15 min at 121°C.
Use:  For the cultivation and maintenance of Desulfobacterium maces-
tii, Desulfomicrobium apsheronum, Desulfomonas pigra, Desulfoto-
maculum species, Desulfovibrio species, and Thermodesulfobacterium
mobile.