Desulfovibrio Medium

[所属分类:培养基配方] [发布时间:2020-6-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Desulfovibrio Medium

培养基配方
Composition per liter:
Solution A.............................................................980.0mL
Solution B..............................................................10.0mL
Solution C..............................................................10.0mL
pH 7.8 ± 0.2 at 25°C
Solution A:
Composition per 980.0mL:
DL -Sodium lactate.......................................................2.0g
MgSO4 ·7H2O.............................................................2.0g
Na2SO4 ..................................................................1.0g
NH4Cl ...................................................................1.0g
Yeast extract............................................................1.0g
K2HPO4 ..................................................................0.5g
CaCl2 ·2H2O.............................................................0.1g
Resazurin ..............................................................1.0mg
Preparation of Solution A: Add components to distilled/deionized
water and bring volume to 980.0mL. Mix thoroughly. Adjust pH to 7.4.
Solution B:
Composition per 10.0mL:
FeSO4 ·7H2O..............................................................0.5g
Preparation of Solution B: Add FeSO4 ·7H2O to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly.
Solution C:
Composition per 10.0mL:
Ascorbic acid .............................................................0.1g
Sodium thioglycolate.......................................................0.1g
Preparation of Solution C: Add components to distilled/deionized
water and bring volume to 10.0mL. Mix thoroughly.
Preparation of Medium: Combine 980.0mL of solution A,
10.0mL of solution B, and 10.0mL of solution C. Mix thoroughly. Ad-
just pH to 7.8. Distribute into tubes or flasks. Autoclave for 15 min at
15 psi pressure–121°C.
Use: For the cultivation and maintenance of Desulfovibrio desulfuri-
cans, Desulfovibrio giganteus, and Desulfovibrio vulgaris.