Desulfovibrio Medium

[所属分类:培养基配方] [发布时间:2020-6-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Desulfovibrio Medium

培养基配方
Composition per 1056.5mL:
(NH4)2SO4 .............................................................5.3g
Sodium acetate.........................................................2.0g
NaCl...................................................................1.0g
KH2PO4 ................................................................0.5g
MgSO4 ·7H2O...........................................................0.2g
CaCl2 ·2H2O ..........................................................0.1g
Na2CO3 solution .....................................................50.0mL
Solution 1...........................................................10.0mL
Solution 2............................................................1.0mL
pH 7.2 ± 0.2 at 25°C
Solution 1:
Composition per liter:
Nitrilotriacetic acid.................................................12.8g
FeCl2 ·4H2O...........................................................0.3g
CoCl2 ·6H2O...........................................................0.17g
MnCl2 ·4H2O ...........................................................0.1g
ZnCl2 ..................................................................0.1g
CuCl2 .................................................................0.02g
H3BO3 .................................................................0.01g
Na2MoO4 ·2H2O.........................................................0.01g
Preparation of Solution 1: Add nitrilotriacetic acid to 500.0mL of
distilled/deionized water. Dissolve by adjusting pH to 6.5 with NaOH.
Add remaining components. Readjust pH to 7.2 with H2SO4 or NaOH.
Add distilled/deionized water to 1.0L.
Solution 2:
Composition per 100.0mL:
Resazurin..............................................................0.2g
Preparation of Solution 2: Add resazurin to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly.
Na2CO3 Solution:
Composition per 100.0mL:
Na2CO3 ................................................................8.0g
Preparation of Na2CO3 Solution: Add Na2CO3 to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize. Gas with 100% N2 for 20 min.
Preparation of Medium: Add components—except Na2CO3 solu-
tion, HCl solution, and Na2S2O4 solution—to distilled/deionized water
and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to
boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–
50°C. Anaerobically and aseptically add 50.0mL of sterile Na2CO3 so-
lution, 5.5mL of sterile HCl solution, and 1.0mL of sterile Na2S2O4 so-
lution. Mix thoroughly. Anaerobically and aseptically distribute into
sterile tubes or flasks.
Use: For the isolation, cultivation, and enrichment of Desulfovibrio
species.