Desulfovibrio Medium (LMG Medium 104)

[所属分类:培养基配方] [发布时间:2020-6-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Desulfovibrio Medium
(LMG Medium 104)

培养基配方
Composition per liter:
Solution A...................................................................980.0mL
Solution B....................................................................10.0mL
Solution C....................................................................10.0mL
pH 7.8 ± 0.2 at 25°C
Solution A:
Composition per 980.0mL:
MgSO4 ·7H2O....................................................................2.0g
DL -Sodium lactate..............................................................2.0g
Yeast extract...................................................................1.0g
NH4Cl ..........................................................................1.0g
Na2SO4 .........................................................................1.0g
K2HPO4 .........................................................................0.5g
CaCl2 ·2H2O ...................................................................0.1g
Resazurin......................................................................1.0mg
Preparation of Solution A: Add components to 980.0mL distilled/
deionized water. Mix thoroughly. Adjust pH to 7.4.
Solution B:
Composition per 10.0mL:
FeSO4 ·7H2O...................................................................0.5g
Preparation of Solution B: Add FeSO4 ·7H2O to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly.
Solution C:
Composition per 10.0mL:
Sodium thioglycolate............................................................0.1g
Ascorbic acid...................................................................0.1g
Preparation of Solution C: Add components to distilled/deionized
water and bring volume to 10.0mL. Mix thoroughly.
Preparation of Medium: Mix 980.0mL solution A, 10.0mL solu-
tion B, and 10.0mL solution C. Adjust pH to 7.8 Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into
sterile Petri dishes or leave in tubes.
Use: For the cultivation of Desulfovibrio desulfuricans subsp. Desul-
furicans and Desulfovibrio vulgaris subsp. Vulgaris.