​Desulfovibrio Marine Medium (DSMZ Medium 163)

[所属分类:培养基配方] [发布时间:2020-6-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Desulfovibrio Marine Medium
(DSMZ Medium 163)

培养基配方
Composition per liter:
Solution A..................................................................980.0mL
Solution B...................................................................10.0mL
Solution C...................................................................10.0mL
pH 7.8 ± 0.2 at 25°C
Solution A:
Composition per 980.0mL:
NaCl..........................................................................25.0g
DL-Na-lactate .................................................................2.0g
MgSO4 ·7H2O...................................................................2.0g
Yeast extract..................................................................1.0g
NH4Cl .........................................................................1.0g
Na2SO4 ........................................................................1.0g
K2HPO4 ........................................................................0.5g
CaCl2 ·2H2O...................................................................0.1g
Resazurin ....................................................................1.0mg
Preparation of Solution A:  Add components to 980.0mL distilled/
deionized water. Mix thoroughly.
Solution B:
Composition per 10.0mL:
FeSO4 ·7H2O.....................................................................0.5g
Preparation of Solution B:  Add FeSO4 ·7H2O to 10.0mL distilled/
deionized water. Mix thoroughly.
Solution C:
Composition per 10.0mL:
Na-thioglycolate.................................................................0.1g
Ascorbic acid ...................................................................0.1g
Preparation of Solution C:  Add components to 10.0mL distilled/
deionized water. Mix thoroughly.
Preparation of Medium: Bring solution A to the boil for a few
minutes. Cool to room temperature while gassing with oxygen-free N2
gas. Add solutions B and C. Mix thoroughly. Adjust pH to 7.8 with
NaOH. Distribute under N2  into anaerobic tubes. During distribution
continuously swirl the medium to keep the grey precipitate suspended.
Autoclave for 15 min at 15 psi pressure–121°C.
Use:  For the cultivation of Desulfovibrio vulgaris, Desulfovibrio desul-
furicans, Desulfovibrio senezii, and Desulfovibrio vietnamensis.