Desulfovibrio magneticus Medium (DSMZ Medium 896)

[所属分类:培养基配方] [发布时间:2020-6-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Desulfovibrio magneticus Medium
(DSMZ Medium 896)

培养基配方
Composition per liter:
Na-fumarate..................................................................0.58g
Na-pyruvate .................................................................0.44g
KH2PO4 .......................................................................0.2g
NH4Cl .......................................................................0.06g
Cysteine-HCl·H2O............................................................0.05g
Vitamin solution.............................................................8.0mL
Trace elements solution......................................................4.0mL
Fe(III)quinate solution......................................................2.0mL
pH 7.0 ± 0.2 at 25°C
Trace Elements Solution:
Composition per liter:
MgSO4 ·7H2O..................................................................3.0g
Nitrilotriacetic acid.........................................................1.5g
NaCl..........................................................................1.0g
MnSO4 ·2H2O..................................................................0.5g
CoSO4 ·7H2O.................................................................0.18g
ZnSO4 ·7H2O.................................................................0.18g
CaCl2 ·2H2O .................................................................0.1g
FeSO4 ·7H2O..................................................................0.1g
NiCl2 ·6H2O................................................................0.025g
KAl(SO4)2 ·12H2O............................................................0.02g
H3BO3 .......................................................................0.01g
Na2MoO4 ·4H2O...............................................................0.01g
CuSO4 ·5H2O.................................................................0.01g
Na2SeO3 ·5H2O...............................................................0.3mg
Preparation of Trace Elements Solution:  Add nitrilotriacetic
acid to 500.0mL of distilled/deionized water. Dissolve by adjusting pH
to 6.5 with KOH. Add remaining components. Add distilled/deionized
water to 1.0L. Mix thoroughly.
Vitamin Solution:
Composition per liter:
Pyridoxine-HCl.............................................................10.0mg
Thiamine-HCl·2H2O..........................................................5.0mg
Riboflavin..................................................................5.0mg
Nicotinic acid..............................................................5.0mg
D -Ca-pantothenate .........................................................5.0mg
p-Aminobenzoic acid.........................................................5.0mg
Lipoic acid.................................................................5.0mg
Biotin......................................................................2.0mg
Folic acid .................................................................2.0mg
Vitamin B 12 ...............................................................0.1mg
Preparation of Vitamin Solution:  Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Sparge
with 80% H2 + 20% CO2 . Filter sterilize.
Ferric Quinate Solution:
Composition per 100.0mL:
FeCl3 ·6H2O.................................................................0.45g
Quinic acid..................................................................0.19g
Preparation of Ferric Quinate Solution:  Add components to
distilled/deionized water and bring volume to 100.0mL. Sparge with
N2 . Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.
Preparation of Medium: Add components, except vitamin solu-
tion and ferric quinate solution, to distilled/deionized water and bring
volume to 990.0mL. Purge medium with N2 gas for 10 min. Mix thor-
oughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.
Aseptically and anerobically add 8.0mL vitamin solution and 2.0mL
ferric quinate solution. Mix thoroughly. Adjust pH to 7.0. Purge medi-
um with N2 gas for 10 min. Under the same atmosphere, aseptically
distribute medium to sterile tubes or bottles.
Use:  For the cultivation of Desulfovibrio magneticus.