Cooked Meat Medium

[所属分类:培养基配方] [发布时间:2020-5-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cooked Meat Medium

培养基配方
Composition per liter:
Beef heart...................................................................454.0g
Proteose peptone..............................................................20.0g
NaCl...........................................................................5.0g
Glucose........................................................................2.0g
pH 7.2 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Finely chop beef heart. Add approximately
1.5g of heart particles to test tubes. Add remaining components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Dis-
tribute into tubes in 10.0mL volumes. Autoclave for 15 min at 15 psi
pressure–121°C. Slowly cool tubes to prevent expulsion of meat parti-
cles.
Use: For the cultivation and maintenance of anaerobic microorgan-
isms.