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Cooked Meat Medium (LMG Medium 140)

[所属分类:培养基配方] [发布时间:2020-5-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cooked Meat Medium
(LMG Medium 140)

培养基配方
Composition per liter:
Heart muscle................................................................454.0g
Peptone......................................................................40.0g
Beef extract.................................................................10.0g
NaCl..........................................................................5.0g
Yeast extract.................................................................5.0g
K2HPO4 .......................................................................5.0g
Glucose ......................................................................2.0g
Resazurin solution...........................................................4.0mL
pH 7.0 ± 0.2 at 25°C
Resazurin Solution:
Composition per 100.0mL:
Resazurin .................................................................0.025g
Preparation of Resazurin Solution: Add resazurin to distilled/
deionized water and bring volume to 100.0mL. Mix thoroughly.
Preparation of Medium: Finely chop beef heart. Add approximately
1.5g of heart particles to test tubes. Add remaining components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Dis-
tribute into tubes in 10.0mL volumes. Autoclave for 15 min at 15 psi
pressure–121°C. Slowly cool tubes to prevent expulsion of meat parti-
cles.
Use: For the cultivation and maintenance of Peptostreptococcus mag-
nus.

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