Congo Red Magnesium Oxalate Agar (CRMOX Agar)

[所属分类:培养基配方] [发布时间:2020-5-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Congo Red Magnesium Oxalate Agar
(CRMOX Agar)

培养基配方
Composition per liter:
Solution 1.................................................................825.0mL
Solution 2..................................................................80.0mL
Solution 3..................................................................80.0mL
Solution 4..................................................................10.0mL
Solution 5...................................................................5.0mL
pH 7.3 ± 0.2 at 25°C
Solution 1:
Composition per 825.0mL:
Pancreatic digest of casein..................................................15.0g
Agar ........................................................................15.0g
Papaic digest of soybean meal.................................................5.0g
NaCl..........................................................................5.0g
pH 7.3 ± 0.2 at 25°C
Preparation of Solution 1: Add components to distilled/deionized
water and bring volume to 825.0mL. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Do
not overheat.
Solution 2:
Composition per liter:
MgCl2 ·6H2O..................................................................50.8g
Preparation of Solution 2: Add MgCl2 ·6H2O to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min
at 15 psi pressure–121°C.
Solution 3:
Composition per liter:
Sodium oxalate ...............................................................33.2g
Preparation of Solution 3: Add sodium oxalate to distilled/deion-
ized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C.
Solution 4:
Composition per 100.0mL:
D- Galactose..................................................................20.0g
Preparation of Solution 4: Add  D- galactose to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
Solution 5:
Composition per 10.0mL:
Congo Red.....................................................................0.1g
Preparation of Solution 5: Add Congo Red to distilled/deionized
water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C.
Preparation of Medium: Aseptically combine 80.0mL of sterile
solution 2, 80.0mL of sterile solution 3, 10.0mL of sterile solution 4,
and 5.0mL of sterile solution 5. Mix thoroughly. Warm to 50°C. Add
this mixture to 825.0mL of cooled, sterile solution 1. Mix thoroughly.
Pour into sterile Petri dishes.
Use: For the cultivation and identification of pathogenic serotypes of
Yersinia enterocolitica. For the determination of whether Yersinia
strains contain the Yersinia virulence plasmid.