Congo Red BHI Agarose Medium (CRBHO Medium) (BAM M41)

[所属分类:培养基配方] [发布时间:2020-5-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Congo Red BHI Agarose Medium
(CRBHO Medium)
(BAM M41)

培养基配方
Composition per liter:
Pancreatic digest of gelatin....................................................14.5g
Agarose.........................................................................12.0g
Brain heart, solids from infusion ...............................................6.0g
Peptic digest of animal tissue...................................................6.0g
NaCl.............................................................................5.0g
Glucose .........................................................................3.0g
Na2HPO4 .........................................................................2.5g
MgCl2 ...........................................................................1.0g
Congo Red solution ............................................................20.0mL
pH 7.4 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes in 20.0mL volumes.
Congo Red Solution:
Composition per 100.0mL :
Congo Red ...................................................................375.0mg
Preparation of Congo Red Solution:  Add Congo Red to
100.0mL of distilled/deionized water. Mix thoroughly. Autoclave for
15 min at 15 psi pressure–121°C. Cool to 25°C.
Use : For the isolation, cultivation, and detection of virulent strains of
Yersinia enterocolitica.