Clostridial Agar

[所属分类:培养基配方] [发布时间:2020-5-7] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clostridial Agar

培养基配方
Composition per liter:
Casein enzymatic hydrolysate .................................................17.0g
Agar .........................................................................14.5g
Glucose .......................................................................6.0g
Papaic digest of soybean meal..................................................3.0g
NaCl...........................................................................2.5g
Na-thioglycolate...............................................................1.8g
Sodium formaldehyde sulphoxylate...............................................1.0g
L -Cystine....................................................................0.25g
NaN3 ..........................................................................0.2g
Neomycin sulfate..............................................................0.15g
pH 7.0 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat while
stirring and bring to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°–55°C. Pour into sterile Petri dishes or distribute
into sterile tubes.
Use: For the selective isolation of pathogenic Clostridia from mixed
flora.