Clostridia Medium

[所属分类:培养基配方] [发布时间:2020-5-7] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clostridia Medium

培养基配方
Composition per liter:
Sodium  L- lactate.............................................................10.0g
Sodium acetate..................................................................8.0g
K2HPO4 .........................................................................0.5g
(NH4)2 ·7H2O...................................................................0.5g
Sodium thioglycolate............................................................0.5g
Yeast extract...................................................................0.5g
MgSO4 ·7H2O....................................................................0.1g
FeSO4 ·7H2O...................................................................0.02g
p-Aminobenzoate.............................................................100.0μg
Biotin .......................................................................0.1μg
pH 6.0–7.0 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.0–7.0.
Distribute into tubes or flasks. Autoclave for 20 min at 15 psi pressure–
121°C.
Use: For the isolation and cultivation of Clostridium species that fer-
ment lactate and acetate.