Chromatium/Thiocapsa Medium

[所属分类:培养基配方] [发布时间:2020-4-29] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Chromatium/Thiocapsa Medium

培养基配方
Composition per 1060.0mL:
KH2PO4 ....................................................................1.0g
NH4Cl .....................................................................1.0g
MgCl2 ·6H2O...............................................................0.5g
Solution A...............................................................20.0mL
Solution B...............................................................20.0mL
Solution C...............................................................10.0mL
Solution D...............................................................10.0mL
Trace elements solution...................................................1.0mL
pH 7.0 ± 0.2 at 25°C
Solution A:
Composition per 100.0mL:
NaHCO3 ...................................................................10.0g
Preparation of Solution A:  Add NaHCO3 to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C.
Solution B:
Composition per 100.0mL:
Na2S·9H2O................................................................10.0g
Preparation of Solution B:  Add Na2S·9H2O to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C.
Solution C:
Composition per 100.0mL:
Na2S2O3 ·9H2O............................................................10.0g
Preparation of Solution C:  Add Na2S2O3 ·9H2O to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Autoclave
for 15 min at 15 psi pressure–121°C.
Solution D:
Composition per 100.0mL:
Sodium malate..............................................................10.0g
Preparation of Solution D:  Add sodium malate to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Autoclave
for 15 min at 15 psi pressure–121°C.
Trace Elements Solution:
Composition per liter:
FeCl3 ·6H2O................................................................2.7g
H3BO3 ......................................................................0.1g
ZnSO4 ·7H2O................................................................0.1g
Co(NO3)2 ·6H2O...........................................................50.0mg
CuSO4 ·5H2O...............................................................5.0mg
MnCl2 ·6H2O ..............................................................5.0mg
Preparation of Trace Elements Solution:  Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components, except solution A, so-
lution B, solution C, and solution D, to distilled/deionized water and
bring volume to 1.0L. Mix thoroughly. Bring pH to 7.0–7.2 with
H3PO4 . Distribute into tubes or flasks. Autoclave for 15 min at 15 psi
pressure–121°C. Aseptically add 0.2mL of sterile solution A, 0.2mL of
sterile solution B, 0.1mL of sterile solution C, and 0.1mL of sterile so-
lution D for each 10.0mL of medium. Mix thoroughly. Use immediate-
ly.
Use: For the cultivation of Chlorobium limnicola and Chromatium spe-
cies.