Chromatium tepidum Medium

[所属分类:培养基配方] [发布时间:2020-4-29] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Chromatium tepidum Medium

培养基配方
Composition per 1001.0mL:
NH4Cl ..................................................................400.0mg
NaCl....................................................................400.0mg
MgSO4 ·7H2O............................................................200.0mg
CaCl2 ·2H2O.............................................................50.0mg
Disodium ethylendiamine-tetraacetate
(Disodium EDTA).........................................................10.0mg
Ammonium acetate (or sodium pyruvate)....................................0.5mg
KH2PO4 ..................................................................0.5mg
NaHCO3  solution .......................................................20.0mL
Na2S·9H2O solution.....................................................20.0mL
Trace elements solution .................................................1.0mL
pH 7.0 ± 0.2 at 25°C
NaHCO3 Solution:
Composition per 20.0mL:
NaHCO3 ...................................................................2.0g
Preparation of NaHCO3 Solution:  Add NaHCO3 to distilled/de-
ionized water and bring volume to 20.0mL. Mix thoroughly. Autoclave
for 15 min at 15 psi pressure–121°C.
Na2S·9H2O Solution:
Composition per 20.0mL:
Na2S·9H2O................................................................1.0g
Preparation of Na2S·9H2O Solution:  Add Na2S·9H2O to dis-
tilled/deionized water and bring volume to 20.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C.
Trace Elements Solutions:
Composition per liter:
Disodium ethylendiamine-tetraacetate
(Disodium EDTA)...........................................................5.2g
FeCl2 ·4H2O..............................................................1.5g
CoCl2 ·6H2O ..........................................................190.0mg
Na2MoO4 ·2H2O ........................................................188.0mg
MnCl2 ·4H2O...........................................................100.0mg
ZnCl2 ..................................................................70.0mg
VOSO4 ·2H2O............................................................30.0mg
NiCl2 ·6H2O............................................................25.0mg
H3BO3 ...................................................................6.0mg
CuCl2 ·2H2O ............................................................2.0mg
Na2WO4 ·2H2O............................................................2.0mg
Preparation of Trace Elements Solutions:  Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components, except NaHCO3  solu-
tion and Na2S·9H2O solution, to distilled/deionized water and bring
volume to 960.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi
pressure–121°C. Aseptically add 20.0mL of sterile NaHCO3  solution
and 20.0mL of sterile Na2S·9H2O solution. Mix thoroughly. Adjust pH
to 7.0. Distribute into sterile screw-capped tubes or bottles so that me-
dium completely fills container.
Use:  For the growth and maintenance of Chromatium tepidum.