Bordetella pertussis Selective Medium with Charcoal Agar Base

[所属分类:培养基配方] [发布时间:2019-12-31] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bordetella pertussis Selective Medium with Charcoal
Agar Base

培养基配方
Composition per 1110.0mL:
Charcoal agar base.........................................1.0L
Horse blood, defibrinated ..............................100.0mL
Cephalexin solution......................................10.0mL
pH 6.7± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
Charcoal Agar Base:
Composition per liter:
Agar....................................................12.0g
Beef extract............................................10.0g
Starch..................................................10.0g
NaCl.....................................................5.0g
Pancreatic digest of casein..............................5.0g
Peptic digest of animal tissue ..........................5.0g
Charcoal.................................................4.0g
Nicotinic acid...........................................1.0mg
Preparation of Charcoal Agar Base: Add components of char-
coal agar base to distilled/deionized water and bring volume to 1.0L.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C.
Cephalexin Solution:
Composition per 10.0mL:
Cephalexin..............................................0.04g
Preparation of Cephalexin Solution: Add cephalexin to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Aseptically add 10.0mL of sterile ce-
phalexin solution and 100.0mL of defibrinated horse blood to charcoal
agar base. Mix thoroughly and pour into sterile Petri dishes.
Use: For the selective isolation and presumptive identification of Bor-
detella pertussis and Bordetella parapertussis. Bordetella pertussis
appears as small, pale, shiny colonies.