Bordetella pertussis Selective Medium with Bordet-Gengou Agar Base

[所属分类:培养基配方] [发布时间:2019-12-31] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bordetella pertussis Selective Medium
with Bordet-Gengou Agar Base

培养基配方
Composition per 1210.0mL:
Bordet-Gengou agar base.....................................1.0L
Horse blood, defibrinated ...............................200.0mL
Cephalexin solution ......................................10.0mL
pH 6.7± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
Bordet-Gengou Agar Base:
Composition per liter:
Agar .................................................20.0g
NaCl...................................................5.5g
Pancreatic digest of casein............................5.0g
Peptic digest of animal tissue.........................5.0g
Preparation of Bordet-Gengou Agar Base: Add components to
1.0L of 1% glycerol solution. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°C.
Cephalexin Solution:
Composition per 10.0mL:
Cephalexin............................................0.04g
Preparation of Cephalexin Solution: Add cephalexin to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Aseptically add 10.0mL of sterile ce-
phalexin solution and 200.0mL of defibrinated horse blood to 1.0L
Bordet-Gengou agar base. Mix thoroughly and pour into sterile Petri
dishes.
Use: For the selective isolation and presumptive identification of Bor-
detella pertussis and Bordetella parapertussis. Bordetella pertussis
appears as small, nearly transparent, “bisected pearl-like” colonies.