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Oxford Medium (BAM M118)

[所属分类:培养基配方] [发布时间:2021-12-8] [发布人:网站管理员2] [阅读次数:] [返回]

Oxford Medium

(BAM M118)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Special peptone.................................................23.0g

LiCl...................................................................15.0g

Agar ..................................................................10.0g

NaCl....................................................................5.0g

Cornstarch...........................................................1.0g

Esculin ................................................................1.0g

Ferric ammonium citrate.....................................0.5g

Antibiotic inhibitor ....................................... 10.0mL

pH 7.0 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from Oxoid.

Supplement Mix:

Composition per 10.0mL:

Cycloheximide....................................................0.4g

Colistin sulfate..................................................0.02g

Fosfomycin .......................................................0.01g

Acriflavine .......................................................5.0mg

Cefotetan..........................................................2.0mg

Ethanol (50% solution)................................. 10.0mL

Preparation of Supplement Mix: Add compo

nents to 10.0mL of ethanol. Mix thoroughly. Filter

sterilize.

Preparation of Medium: Add components, ex

cept supplement mix, to distilled/deionized water

and bring volume to 990.0mL. Mix thoroughly. Gen

tly heat and bring to boiling. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 50°C. Aseptically

add 10.0mL of sterile supplement mix. Mix thor

oughly. Pour into sterile Petri dishes or distribute into

sterile tubes.

Use: For the isolation and cultivation of Listeria

monocytogenes from specimens containing a mixed

bacterial flora.